Name: NAD+
Brand: Hati Peptides
SKU: NAD-500MG
Price: 42.29 GBP
Availability: OutOfStock

Question 1

What is NAD+ and how does it work in research models?

Accepted Answer

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme found in all living cells. In research models, it serves as an essential electron carrier in metabolic redox reactions and as a substrate for enzymes involved in cellular signalling, DNA repair, and gene regulation. The molecule exists in two forms: NAD+ (oxidised) and NADH (reduced). The NAD+/NADH ratio is a critical indicator of cellular metabolic state. NAD+ levels decline with age, falling by approximately 50% between age 20 and 60 in human tissues, making it a key subject in ageing research.

Question 2

How do I reconstitute NAD+ for laboratory use?

Accepted Answer

Reconstitute lyophilised NAD+ with sterile water or phosphate-buffered saline (PBS). The coenzyme is freely soluble in aqueous solutions. Typical stock concentrations range from 10–100 mM depending on assay requirements. Store lyophilised powder at −20°C, protected from light; reconstituted solution at 2–8°C, use within 24 hours. NAD+ is unstable in solution; prepare fresh for each assay. Protect from light during storage and use. Optimal stability at pH 7.0–8.0. Reconstituted solutions may turn yellow if contaminated with NADH; discard if colour change is observed.

Question 3

What purity standard is recommended for NAD+ research?

Accepted Answer

Research-grade NAD+ should be ≥99% pure. The oxidised form (NAD+) should be confirmed by spectrophotometry (absorbance at 260 nm) and mass spectrometry (molecular weight 663.4 Da). The reduced form (NADH) has different absorbance properties (340 nm) and should be absent in NAD+ preparations. Batch-specific Certificates of Analysis should document purity, identity, and absence of NADH contamination. Fresh reconstitution is essential as NAD+ degrades in solution.

Question 4

Is NAD+ legal for research in the UK?

Accepted Answer

Yes. NAD+ is not a controlled substance under the UK Misuse of Drugs Act 1971 and is not scheduled under the Psychoactive Substances Act 2016. It is classified as a research biochemical for laboratory use and is not licensed as a medicine by the MHRA. Research institutions should ensure compliance with institutional ethics approvals and standard laboratory safety protocols.

Question 5

What cellular models are used for NAD+ research?

Accepted Answer

Standard cellular models include: (1) NAD+/NADH ratio assays using enzymatic cycling or fluorescent probes; (2) sirtuin activity assays with recombinant enzymes and acetylated substrates; (3) PARP activity assays measuring poly(ADP-ribose) synthesis; (4) mitochondrial respiration studies using Seahorse respirometry; (5) cellular NAD+ depletion models using NAMPT inhibitors (FK866); and (6) ageing models examining senescence markers in response to NAD+ modulation. The coenzyme is studied in the context of metabolic flux, epigenetic regulation, and DNA repair.

NAD+

NAD+

Research Overview

Areas of Interest

Technical Specifications

Certificate of Analysis